Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells

We aimed to evaluate the effect of activated platelet-rich plasma (PRP) on proliferation and chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BMSCs). Six mature male rabbits were included in this study. PRP was obtained by two-step centrifugation from whole blood, it using CaCl2 solution. BMSCs isolated proliferated marrow characterized flow cytometry. Passage 3 cultured high-glucose Dulbecco’s modified Eagle’s medium (HG-DMEM) with four different compositions for consecutive 7 days, including 10% fetal bovine serum, 5% PRP, 15% PRP. Cell counting assays performed cell BMSCs. ( 5 × 10 cells/well 6-well plates) induced conditions 21 days evaluation, commercial (control), (HG-DMEM+5% PRP), (HG-DMEM+10% (HG-DMEM+15% PRP). The gene expression levels ACAN, COL2A1, SOX9 pellets detected. Morphological pathological assessments blind observer. After purifying, percentages CD105(+)/CD34(−) CD44(+)/CD45(−) 96.5% 92.9%, respectively. enhanced all groups, revealed more significant outcome than others day 5. lower three groups control group, but ACAN higher group groups. Histological examinations showed that PRP-treated had regular appearance, larger size, abundant extracellular matrix or still inferior medium. In conclusion, our results show may enhance rabbit However, have limited comparison culture.